Coding

Part:BBa_K3977000

Designed by: Yilong Xu   Group: iGEM21_SCU-China   (2021-10-01)


Cas12j-D394A (or dCasΦ), for more see 2021 SCU-China wiki page result and model

from complex CRISPR/Cas, 700aa

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 2178
    Illegal NgoMIV site found at 2220
    Illegal AgeI site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1447
    Illegal SapI site found at 1240



Contribution by SVCE-CHENNAI

The three dimensional structure of the protein was predicted using SWISS-MODEL. The model was validated using Ramachandran plot analysis. It was observed that 94 % of the residues were under the favoured region. This confirms the reliability of the predicted protein model Figure 1.


k3977000-rc-plot.png

Figure 1: Ramachandran Plot for the modelled protein structure


k3977000-model-str.png


Figure 2: 3D Structure of modelled protein

[edit]
Categories
//function/crispr/cas
Parameters
biology