Conjugation

Part:BBa_K3946006

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-21)


L2NC silica tag+term (CT)

Part BBa_K3946002 with the terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 486
    Illegal suffix found in sequence at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 486
    Illegal NheI site found at 404
    Illegal NheI site found at 427
    Illegal SpeI site found at 695
    Illegal PstI site found at 709
    Illegal NotI site found at 492
    Illegal NotI site found at 702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 486
    Illegal suffix found in sequence at 695
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 486
    Illegal XbaI site found at 501
    Illegal SpeI site found at 695
    Illegal PstI site found at 709
    Illegal NgoMIV site found at 2327
    Illegal NgoMIV site found at 2456
    Illegal NgoMIV site found at 2950
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 522
    Illegal BsaI.rc site found at 675
    Illegal SapI.rc site found at 3748


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Categories
Parameters
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