Terminator

Part:BBa_K3946005

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-21)


Car9 silica tag+term (CT)

Part BBa_K3946001 with BBa_K3946043.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 164
    Illegal suffix found in sequence at 372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 164
    Illegal NheI site found at 82
    Illegal NheI site found at 105
    Illegal SpeI site found at 373
    Illegal PstI site found at 387
    Illegal NotI site found at 170
    Illegal NotI site found at 380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 164
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 164
    Illegal suffix found in sequence at 373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 164
    Illegal XbaI site found at 179
    Illegal SpeI site found at 373
    Illegal PstI site found at 387
    Illegal NgoMIV site found at 2005
    Illegal NgoMIV site found at 2134
    Illegal NgoMIV site found at 2628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 200
    Illegal BsaI.rc site found at 353
    Illegal SapI.rc site found at 3426


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