RNA

Part:BBa_K3907003

Designed by: Laura Keffer-Wilkes   Group: iGEM21_Lethbridge_HS   (2021-10-12)


siRNA_scramble against Clpp

This construct contains 26 repeating copies of a 19 bp fragment that is a scrambled version of BBa_K3907002 against the Arabidopsis gene ClpP. The scramble sequence can be used as a control to test the efficacy of the original siRNA construct. The DNA is to be used as a template to create siRNA fragments using RNase H and DNA chimer splints as per Feyrer et al. (2020).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
    Illegal NotI site found at 337
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None