Part:BBa_K3842005

pPICZαA(plasmid_backbone)

It's a plasmid's backbone of pPICZαA

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1215
  Illegal XbaI site found at 1235
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1215
  Illegal NotI site found at 1221
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1215
  Illegal BglII site found at 1
  Illegal BamHI site found at 1641
  Illegal XhoI site found at 1191
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1215
  Illegal XbaI site found at 1235
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1215
  Illegal XbaI site found at 1235
  Illegal NgoMIV site found at 2399
  Illegal NgoMIV site found at 2460
  Illegal AgeI site found at 1360
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1624
  Illegal BsaI.rc site found at 1823
  Illegal BsaI.rc site found at 2821