Part:BBa_K3838234
YH-BSH
Bilesalthydrolase (BSH), responsible for hydrolysis in combination with bile salts to produce free bile acids and amino acids. BSH activity in gastrointestinal tract was mainly from Firmicutes (30%), Bacteroidetes (14.4%) and actinobacteria (8.9%). Impaired microbial enzyme activity in IBD patients leads to impaired bile acid metabolism, characterized by the inability to hydrolyze, transform, and desulphurize bile salts. Microbial BSH activity removes glycine or taurine molecular coupling to produce unbound bile acids. Abnormal intestinal bile salt metabolism enhances the inflammatory response of the intestinal epithelium, which worsens IBD. It has been documented that the ingestion of probiotics with BSH activity can promote the bioconversion of intestinal bile salts, thereby alleviating the inflammatory response induced by high doses of intestinal bile salts. Therefore, we selected heterologous expression of BSH gene from Lactobacillus acidophilus.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal BglII site found at 1046 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24 - 1000COMPATIBLE WITH RFC[1000]
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