Coding

Part:BBa_K3831052

Designed by: Barbora Hrnčířová   Group: iGEM21_Brno_Czech_Republic   (2021-10-14)


PduB - reverse complement

This is one of the BMC (bacterial microcompartment) shell proteins from the Pdu operon of the bacteria Parageobacillus thermoglucosidasius which is closely related to B. subtilis. Heterologous production and assembly of Pdu BMC in B. subtilis has been demonstrated in a study by Wade et al. (2019), where they have identified five essential shell proteins. This is the second of them. See parts BBa_K3831000, BBa_K3831002, BBa_K3831003, and BBa_K3831004 to find out the information on the other BMC shell proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 266
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//function/structures
Parameters
chassisBacillus subtilis