Plasmid_Backbone
Part:BBa_K382001
Designed by: Christina Agapakis Group: iGEM10_Harvard (2010-10-26)
pORE Open Serices Vector with BioBrick MCS (Kan resistance)
Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has no promoter or reporter. Conforms to Silver BioBrick Assembly Standard 21.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4759
Illegal XbaI site found at 4774
Illegal SpeI site found at 4780
Illegal PstI site found at 4794 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4759
Illegal SpeI site found at 4780
Illegal PstI site found at 4794
Illegal NotI site found at 4765
Illegal NotI site found at 4787 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4759 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4759
Illegal suffix found at 4780 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4759
Plasmid lacks a suffix.
Illegal XbaI site found at 4774
Illegal SpeI site found at 4780
Illegal PstI site found at 4794
Illegal NgoMIV site found at 625
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 2181
Illegal NgoMIV site found at 2772
Illegal NgoMIV site found at 6642
Illegal NgoMIV site found at 7277
Illegal AgeI site found at 341 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 7126
Illegal SapI.rc site found at 7336
[edit]
Categories
Parameters
//chassis/eukaryote/athaliana
//plasmid/chromosomalintegration
//plasmid/chromosomalintegration
chassis | Agrobacterium tumefaciens |
origin |