RBS

Part:BBa_K3796212

Designed by: Yiwei Wang   Group: iGEM21_CAU_China   (2021-09-24)

A strong strength RBS

This is a strong strength RBS for C.glutamicum ATCC 13032,which was used in the overexpression of ppc in our project.


Biology

This is a strong strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can increase gene expression by promoting translation in Corynebacterium glutamicum.

Usage

By applying this strong strength RBS, overexpression of genes can be made through regulation on the translation level. It has been verified that this RBS can realize overexpression in Corynebacterium glutamicum. However, application in other species is not clear yet.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None