Part:BBa_K3766035

Synthetic RBS designed for LacI

This part is a synthetic RBS designed for LacI (BBa_K091121 and BBa_K1088018) using the Salis Lab / De Novo DNA RBS Calculator v2.1 [1-6].

Usage and Biology

This RBS was designed for and used to drive the expression of LacI (BBa_K091121 and BBa_K1088018) under the control of the T7 promoter (BBa_K2150031) in the composite part BBa_K3766190.

References

[1] Salis HM. The ribosome binding site calculator. Methods in Enzymology (2011) 498: 19–42.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nature Biotechnology (2009) 27: 946–950.

[3] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Research (2014) 42: 2646–2659.

[4] Espah Borujeni A, Salis HM. Translation initiation is controlled by RNA folding kinetics via a ribosome drafting mechanism. Journal of the American Chemical Society (2016) 138: 7016–7023.

[5] Espah Borujeni A, Cetnar D, Farasat I, Smith A, Lundgren N, Salis HM. Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences. Nucleic Acids Research (2017) 45: 5437–5448.

[6] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]