RNA

Part:BBa_K3714001

Designed by: Yuxin Lu   Group: iGEM21_SJTU-BioX-Shanghai   (2021-09-29)


Theophylline biosensor AAG-AAAAA

The template of a theophylline biosensor. Cleave itself without theophylline. Two A-rich sequences were attached at both terminal to minimal the unwanted interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
K3714001.png
Figure.1 | Verification of biosensor theo-AAG/AAAAA. An in vitro transcription system was employed to test if this biosensor can response theophylline signal. The product was run on a denatured PAGE.


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