Primer

Part:BBa_K3658016

Designed by: Yujeong Oh   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-26)


LAMP BIP for Batrachochytrium dendrobatidis (Bd) ITS region

Backward internal primer (FIP), or b1c-b2, used for LAMP amplification of Bd ITS region. LAMP stands for Loop-mediated AMPlification and it is a technique that amplifies DNA at a constant temperature lower than for PCR. The ITS (internal transcribed spacer) region is highly conserved and widely used as the DNA barcode region in fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/part
//primer/part/amplification
Parameters
None