Terminator

Part:BBa_K3654006

Designed by: Cheny Yang   Group: iGEM20_KSA_KOREA   (2020-10-27)


A transcriptional terminator for CYC1

The terminator/polyadenylation site found at the end of the CYC1 gene from S. cerevisiae. The CYC1 terminator yields a stable RNA even when close to the promoter. In this project, CYC1 terminator was utilized to transcriptional termination of Laccase and DyP gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None