RNA
SARSi-R1

Part:BBa_K3626000

Designed by: Salma Abou Elhassan   Group: iGEM20_AUC-EGYPT   (2020-10-11)


SARS-CoV-2 Replicase-targeting siRNA

This sequence comprises 21‐kb of the genome encoding two polyproteins (encoded by ORF1a and ORF1b) that undergo cotranslational proteolytic processing. It is a replicase-targeting siRNA sequence that corresponds to the nucleotide location 629-651 of SARS-CoV-2 for ORF1ab gene. ORF1ab encodes for the replicase proteins that are responsible for SARS-CoV-2 replication and making more viral copies. This siRNA sequence works on inhibiting that viral replication by binding to the complementary strand of replicase mRNA of SARS-CoV-2, cutting it and thus blocking its translation, resulting in inhibition of virus replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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