RNA

Part:BBa_K3602007

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs6983267-G Target Cas13a

This part includes the reverse complementary RNA sequence for the SNP rs6983267. This SNP is associated with increased risk for prostate cancer and can therefore be used for risk assessment tests for prostate cancer. The sequence is the risk allele of this SNP, while BBa_K3602006 is the non-risk allele. This sequence binds complementary to the rs6983267-G SNP sgRNA (BBa_ K3602009) which can be used together with cas13a (BBa_K3602001). The part is the reverse complementary to the BBa_K3602016 part.

The part has a T7 promoter, in the beginning, enabling it for in vitro transcription from DNA to mRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None