Terminator

Part:BBa_K3568009

Designed by: MENG XU   Group: iGEM20_XH-China   (2020-10-22)


Terminator.png

biologyrrn B T1
chassisrrn B T1 transcriptional terminator
directionForward and Reverse
forward_efficiency96%
reverse_efficiencyN/A

rrnB T1 and T2 transcriptional terminators

  • The termination region of this terminator in Escherichia coli is large and complex. It contains two regions (T1 and T2) with typical factor-independent terminator-like sequences, two additional inverted repeats (lR1 and IR2) and a pair of direct repeats. The whole region is frequently employed as an efficient terminator of transcription in several cloning vectors. In the pDONR vector series it protects the cloned gene from expression by vector-encoded promoters, thereby reducing possible toxicity.

Secondary Structure

Mfold-K3568009-1.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
//terminator/single
Parameters
biologyrrn B T1
chassisrrn B T1 transcriptional terminator
directionForward and Reverse
forward_efficiency96%
reverse_efficiencyN/A