Part:BBa_K3497003
a highly glycosylated protein embedded in the viral envelope of SARS-CoV-2
The spike(S) protein is a highly glycosylated protein embedded in the viral envelope of coronaviruses. The spike protein of the SARS-CoV-2 plays a crucial role in penetrating host cells and initiating infection. There are two function domains of the S protein, the S1 and the S2, and the S1 domain has the specialized ability to bind with the ACE2 of the targeted cells. After the connection of the S protein on the surface of SARS-CoV-2 and the extracellular domain of the ACE2 on the targeted cells, the extracellular domain of the ACE will be lysed and the remaining SARS-CoV-2-ACE complex will be internalized on the assistant of the Clathrin. After the fusion between the lipid bilayer in the SARS-CoV-2 and the one in the targeted cell, the RNA of the SARS-CoV-2 can finally be released and inserted to the targeted gene sequence to conduct virus replication. Accounting the critical function of the S protein plays in the SARS-CoV-2 infection process, we are determined to conduct a replication-defective pseudo- SARS-CoV-2 by expressing the S protein in the replication-defective vesicular stomatitis virus (Delta-G-VSV pseudovirus). The sequence of the spike protein is from GenBank (YP_009724390), and we ordered the DNA from a synthesis company.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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