RNA

Part:BBa_K3467003

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-21)


CHS dsRNA sequence

CHS is a coding sequence of chitin synthase with 5116bp cDNA. CHS has a critical function in locust. CHS gene will express in all the developmental stages and is responsible for chitin synthesis in cuticle and trachea of locust. We designed the dsRNA sequence of CHS by using specific program(https://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl) and find a 669bp length of CHS dsRNA for RNAi.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 584
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 584
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 584
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 584
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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