RNA

Part:BBa_K3425038

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-17)


Qβ5' recognition sequence of Qβ replicase

BBa_K3425038 is the recognition sequence of the Qβ viral replicase at the 5'end of its RNA templates. BBa_K3425038 sequence was retrieved from the publication [1]. The sequence of this part is highly similar, but non-identical to the MDV downstream.

About Qβ replicase

Qβ replicase is an RNA-dependent RNA polymerase of Qβ phage. Functional enzyme consists of four parts, where only a single component - the β-subunit - is of viral origin. The remaining three components are ribosomal protein S1 and the translation elongation factors EF-Tu and EF-Ts. For its function, Qβ replicase requires the presence of specific recognition sequences at the both ends of its RNA template, such as QB5' and QB3' (BBa_K3425039) or MDV upstream and downstream.


Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/bacteriophage
//chassis/prokaryote/ecoli
//classic/rna/uncategorized
//direction/forward
Parameters
chassisE. coli
device_typeRNA functional element
functionRNA amplification