RNA

Part:BBa_K3406017

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-10-27)


PsmCas13b-SARS-CoV-2-crRNA

The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas13 protein, and different Cas13 proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to PsmCas13b and SARS-CoV-2, which could combine with PsmCas13b protein and guide it to target SARS-CoV-2 sequence, thus activating the collateral cleavage of PsmCas13b protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None