RNA

Part:BBa_K3356018

Designed by: Luke Weir   Group: iGEM20_Nottingham   (2020-10-27)


Synthetic guide RNA sequence 2 for tetR integration upstream of spoIID


Sequence coding for synthetic guide RNA 2 to direct the cas9 mediated insertion of the TetR inducible promoter upstream of the spoIID gene. This is to create a conditional sporulating strain which will only sporulate in the presence of the inducer, anhydrotetracyline (aTc), which is a derivative of tetracycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None