Part:BBa_K332031

This device is a synthetic gene that generates tetR, a protein regulates Ptet, when environment's temperature above 37° C.

The thermosensing function is controlled by the 37°C induced RBS (BBa_K115002) and the tetR gene(BBa_C0040).

By using different temperature-regulated ribosome binding sites, we can modify the switching temperature (37°C ) of this device according to different needs.

Since the tetR gene can repress the pTet promoter (BBa_R0040), the downstream genes of pTet, such as the target gene , can also be repressed.

While the switching temperature is achieved in the incubator, pTet as well as its downstream genes will be switched off.

In this condition(above 37° C), almost none of the target protein can be detected.

However, pTet and its downstream genes will not be switched off below the switching temperature. In other words, we can observe a great amount of the crystal protein as well as the green fluorescent protein only in sub 37°C environment.