RBS

Part:BBa_K3288005

Designed by: Joshua Nam   Group: iGEM19_SIS_Korea   (2019-10-15)


Cdog (strong rbs)

We constructed a total of 12 expression units by selecting four promoters and three RBS candidates to find a combination of a strong constitutive promoter and a strong RBS capable of continuously expressing a gene of interest in Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Chen, J., Zhu, X., Tan, Z., Xu, H., Tang, J., Xiao, D., & Zhang, X. (2014). Activating C 4-dicarboxylate transporters DcuB and DcuC for improving succinate production. Applied microbiology and biotechnology, 98(5), 2197-2205.


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