Composite

Part:BBa_K3273009

Designed by: Bruno Batista, Fernando de Souza, Isabelle Taira, Lucas Boldrini   Group: iGEM19_Sao_Carlos-Brazil   (2019-10-14)


Composite HXT6 Bax

This composed part has the Hxt6 promoter BBa_K3273003, expressed in low glucose concentration, followed by the Kozak sequence BBa_J63003, Mammalian Bax apoptotic protein BBa_K3273007 and the BBa_K2637017 terminator. It is a kill-switch regulated by glucose concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1337
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2034
    Illegal XhoI site found at 152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1337
    Illegal NgoMIV site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None