Composite

Part:BBa_K3273008

Designed by: Bruno Batista, Fernando de Souza, Isabelle Taira, Lucas Boldrini   Group: iGEM19_Sao_Carlos-Brazil   (2019-10-14)


Composite ADH2/TDH3 nucA

This part is composed of the BBa_K3273004 promoter, which is expressed in low glucose concentration, followed by a Kozak sequence BBa_J63003, then Serratia marcescens nucA nuclease BBa_K3273001 and the BBa_K2637017 terminator. It is a molecular kill switch activated in low glucose concentrations, that could prevent the DNA to be available to environmental organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None