Coding

Part:BBa_K3221102

Designed by: Liu Yuxi   Group: iGEM19_SCU-China   (2019-09-30)


CNS1-linker-CNS2 coding sequence.

CNS1 and CNS2 are two enzymes responsible for the synthesis of COR(cordycepin). The two enzymes were found by CAS Key Laboratory of Insect Developmental and Evolutionary Biology. It was described that Cns2 catalyzes 3'-AMP to 2'-C-3'-dA and then CNS1 catalyzes 2'-C-3'-dA into COR. So this part is able to express enzymes needed for catalyze 3'-AMP into COR. This part is composed of coding sequences of CNS1 and CNS2, which are linked into a fusion protein by a linker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1288
    Illegal BglII site found at 1875
    Illegal BglII site found at 2293
    Illegal BamHI site found at 2377
    Illegal XhoI site found at 3484
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3490
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
    Illegal AgeI site found at 1354
    Illegal AgeI site found at 3553
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//function/biosynthesis
Parameters
None