Coding
hspCas9

Part:BBa_K3120003

Designed by: Ruoyi Xue   Group: iGEM19_BM-AMU   (2019-08-10)


A human codon-optimized SpCas9

humanized S.pyogenes Cas9

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 2179
    Illegal NgoMIV site found at 2252
    Illegal NgoMIV site found at 2737
    Illegal NgoMIV site found at 3646
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/cre
Parameters
None