Composite

Part:BBa_K3096055

Designed by: Patrick Mueller, Jakob Keck, Lina Widerspick, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)


Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter

The Expression of the Cascade ABCDE is under the control of the AraC (pBAD) promoter and ist expression can hence be blocked when AraC is present. By regulating the transcription of Cascade ABCDE this Part can be used for a CRISPR/Cas3 based kill-switch where only when certain conditions are met Cascade ABCDE will be transcribed (absence of AraC).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None