Composite

Part:BBa_K3096054

Designed by: Patrick Mueller, Lina Widerspick, Jakob Keck, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)


Cas3 Nuclease under the control of the AraC promoter pBAD

The Expression of the nuclease Cas3 is under the control of the AraC (pBAD) promoter and ist expression can hence be blocked when AraC is present. By regulating the transcription of Cas3 this Part can be used for a CRISPR/Cas3 based kill-switch where only when certain conditions are met the Cas3 will be transcribed (absence of AraC).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None