Translational_Unit

Part:BBa_K3071018

Designed by: Leung Hei Man   Group: iGEM19_Hong_Kong-CUHK   (2019-10-13)


Xcc RpfC with HA-tag and RBS

This composite part contains the Sensory/regulatory protein RpfC from Xanthomonas campestris pv. camperstris((BBa_K3071000) with the Ribosome binding site (BBa_J61104) and C-terminus HA-tag with a double stop codon (BBa_K3071015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 321
    Illegal AgeI site found at 1153
    Illegal AgeI site found at 1632
    Illegal AgeI site found at 1716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 370
    Illegal BsaI.rc site found at 234


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Categories
Parameters
None