RBS

Part:BBa_K3027005

Designed by: Ombeline Rossier   Group: iGEM19_GO_Paris-Saclay   (2019-10-21)


RBS in pBAD24-MoClo (Golden Gate compatible)

This is the RBS sequence present on pBAD24 and its derivative, pBAD24-MoClo. The latter is a plasmid that we designed to be compatible with Golden Gate assembly according to iGEM standards for CDS cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


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