RNA

Part:BBa_K3001025

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-19)


snR30 + RNA Mango II

Purification tag for the RNA cleaving enzyme Cas13a isolated from Leptotrichia buccalis . Contains a His tag, Twin strep tag, and a SUMO tag. Please see part BBa_K3001028 for experience using this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 594
    Illegal SpeI site found at 134
    Illegal PstI site found at 1053
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 529
    Illegal SpeI site found at 134
    Illegal PstI site found at 1053
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 546
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 594
    Illegal SpeI site found at 134
    Illegal PstI site found at 1053
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 594
    Illegal SpeI site found at 134
    Illegal PstI site found at 1053
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024


[edit]
Categories
Parameters
None