Part:BBa_K3001017
Lba crRNA targeting RNA Mango with T7 promoter
This CRISPR RNA (crRNA) is designed to interact with Cas13a isolated from Lachnospiraceae bacterium (Lba). It is designed to target snR30-RNA Mango II RNA transcript. It is designed to be in vitro transcribed using T7 polymerase. This construct was synthesized in pUC19.
wetlab data
CRISPR RNA (crRNA) is a crucial aspect of the CRISPR Cas13a system. It seeks out the target RNA sequence so that the Cas13a can cleave the RNA. Our crRNA sequences were synthesized by IDT into the plasmid pUC19. Then we successfully PCR amplified the DNA out of the pUC19 plasmid.
Figure 1. 10% DNA PAGE of crRNA products for Lba and Lbu. Left to right: lane 1: empty; lane 2: 100 bp ladder (Thermo Scientific); lane 3: Lbu 57.1; lane 4: Lbu 56; lane 5: Lbu 55; lane 6: Lbu 54; lane 7: Lba 57.1; lane 8: Lba 56; lane 9: Lba 55; lane 10: Lba 54.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 67
- 1000COMPATIBLE WITH RFC[1000]
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