RNA

Part:BBa_K3001010

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lbu crRNA targeting GFP

This CRISPR RNA (crRNA) is designed to interact with Cas13a isolated from Leptotrichia buccalis (Lbu). It is designed to target a segment of GFP mRNA transcript. It is designed to be inducibly expressed under a T7 promoter and was used for in vivo experiments in E. coli BL21(DE3). Experiments were conducted in the plasmid pUC19.Please see part BBa_K3001011 for experience using this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None