Part:BBa_K2896001
Thylakoid-bound SuperNova (for S. elongatus PCC 7942)
This is a membrane-bound version of SuperNova protein, originally introduced by iGEM 2014 Carnegie Mellon team. Link: BBa_K1491017
SuperNova is a photosensitizing protein, which produces Reaction Oxygen Species (superoxide and singlet oxygen) under irradiation of 500-600nm wavelength light. It is a monomeric version of another photosensitizing protein called KillerRed. There are several advantages over KillerRed, SuperNova does not oligomerize inside the cell and does not interfere with mitotic cell division. This advantage allows performing targeted protein inactivation inside the cell by fusion to the target protein without loss of target protein function. [1]
The second application that seems very simple and powerful is light controlled safety system. SuperNova can be utilized as a killer switch for genetically modified organisms. Under irradiation of 500-600nm wavelength light, SuperNova will generate ROS and kill the cell. Its phototoxicity was proven in bacterial and mammalian cells. [1]
1. Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., ... & Ayabe, T. (2013). SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3, 2629.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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