RNA

Part:BBa_K2877028

Designed by: Andrew D Ellington   Group: iGEM18_Austin_LASA   (2018-10-11)


Rev crRNA 4 (LbCas12a)

This is a crRNA sequence for Rev. It includes a hairpin sequence for LbCas12a and as such should be used with LbCas12a. Following the hairpin sequence is a spacer sequence from Rev that serves as the main method in which Cas12a identifies our viral DNA. These sequences can either be ordered as RNA oligos or transcribed via T7 in vitro transcription by adding a T7 promoter at the start of the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None