Coding

Part:BBa_K2760023

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-11)


NsrR Optimized for both L. rhamnosus GG & E.coli

It is the NsrR - NO(x) sensitive pYear, pNirk repressor protein but modified for expression in L.rhamnosus GG. This protein binds to the Pyear promoter sequence inhibiting expression, however when Nitric oxide binds to this protein, it releases the promoter sequence, thus genes downstream can be expressed. This system is present in E.coli, in the yeaR-yoaG operon, however this part was designed to test its performance on another host (L. rhamnosus GG).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/immune_regulation/devices
Parameters
None