RNA

Part:BBa_K2725014

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)


J23102 - supD - B1006

An serine amber suppressor tRNA gene downstream of J23102 Anderson promoter (strength 0.82). Due to high expression levels this part, when co-transformed, should allow expression of protein coding genes with high numbers of amber stop codons.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 80


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Parameters
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