Part:BBa_K2675016

Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX full promoter (BBa_K2675020) or its derivatives (BBa_K2675023, BBa_K2675024, BBa_K2675025) in the composite parts BBa_K2675050, BBa_K2675053, BBa_K2675054, BBa_K2675055, BBa_K2675057, BBa_K2675060, BBa_K2675063, BBa_K2675064 and BBa_K2675065.

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	202865,54
dG_total (kcal/mol)	:	-11,34
dG_mRNA_rRNA (kcal/mol)	:	-21,58
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-13,78
Accuracy (warnings)	:	OK

REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]