Part:BBa_K266007

Complex QS -> LuxI & LasI circuit

Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.

Widespread quorum sensing (QS) enables communication among bacteria and plays a critical role in bacterial virulence. Lux R/I and Las R/I systems can trigger crosstalk in certain bacteria, trigger biofilm formation, and develop synthetic ecosystems. Gram negative bacteria generally use the LuxI/LuxR type systems mediated by acylated homoserine lactones (AHL). For QS crosstalk, functional interactions between QS components are not naturally paired.

LuxI with LasI, as well as LasR with LasI can activate pLux. The QS regulator-autoinducer pairs are not orthogonal, and there is crosstalk between them. Crosstalk is what reveals the principles of bacterial decision making and survival strategies for both natural and synthetic systems.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1771
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 813
  Illegal BglII site found at 2533
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 375
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1492