Part:BBa_K2656016

LuxR TF Coding Sequence

Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656016 is the transcription factor LuxR coding sequence BBa_C0062 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It acts on the lux promoters when activated by Acyl-homoserine-lactone. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

This part used to construct the composite part BBa_K2656114. Composite part BBa_K2656114 was then used together with BBa_K2656116 in order to characterize the pLux repressible promoter BBa_K2656002.

By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol] and inducing at different AHL concentrations, we have obtained the following experimental measures (in blue dots). These measurements were compared with our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive AHL-LuxR model] to test it.

Figure 3. AHL induction experiment and comparison with our model simulation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]