Part:BBa_K2644124
GGG+crRNA pDsRed-Monomer-N1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This biological part is specially designed to target a RED plasma. The principle behind this is that CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as components of bacterial adaptive immune systems like CRISPR-Cas9. We designed the crRNA which is able to combine with the Cas12a protein and recognize the target DNA. According to this principle, we designed a crRNA that matched with one fragment of RED plasma., which brings Cas12a to its target, specifically cleaves the RED plasma. Through this method, we are theoretically capable of targeting any single base mutation on ctDNA which are dispersed in people’s blood.
Cleaving experiment
Figure 1.Figure 5. Optimization of cleavage protocol. (A) Line 1, GFP plasmid. Line 2, cleavage plasmid with BamH1 enzyme. Line3-7, cleavage according to table B. (B) Experiment design.
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