RNA

Part:BBa_K2611009

Designed by: Yumei Yang   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA (J23100)

This part contains a sgRNA generator with a sgRNA sequence that can specifically bind to the reverse sequence of J23100 promoter. Our project is to use sgRNA sequences to programmably regulate the expression of different genes in E.coli genome, with methods like AND gates and OR gates. So we need to find several different sgRNA sequences that can bind to different parts of one same gene. This part is one of such sgRNA sequences we designed that binds to J23100 promoter, so, with dCas 9 protein, it can regulate the expression of genes with J23100 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biology
negative_regulators