RNA

Part:BBa_K2558007

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)


gRNA targeting lux pR and RiboJ

gRNA targeting lux pR and RiboJ is a sequence used in our NEON CRISPRi Safety Catch device (K2558214, K2558215), whose function is to guide dCas9 to the lux pR promoter and therefore inhibit the expression of downstream genes (luxI and GFP). Even though the lacI on the Safety Catch is also controlled by luxpR, this design of gRNA whose targeting sequence also includes part of the sequence of RiboJ allows us to make the lux pR in Safety Catch, which is not followed by RiboJ, unaffected by CRISPRi. Therefore lux pR on Safety Catch can still be activated by luxR-AHL complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr
Parameters
None