RNA

Part:BBa_K2510055

Designed by: Joseph Ryan   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


49xCAG Repeats

49xCAG Repeats

Usage and Biology

The 49xCAG sequence was built using two oligonucleotides: 10xCAG and 10xCTG. Through testing a variety of oligonucleotide concentrations and testing a different of PCR conditions, a specific protocol was built to synthesise various lengths of DNA containing CAG repeats.

The 49xCAG repeats were used in FA-FB System where the FA component is expressed an MS2 aptamer, which is a specific bacteriophage binding site that connects an MS2 binding domain. By expressing the CAG repeat sequence with an MS2 binding domain, specific binding can occur on the RNA aggregation. Thus, this creates a localization of FA in the cell.

RNA_organelle.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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