RBS

Part:BBa_K2448019

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


RBS (pET like)

This part is a strong RBS derived from the one present in the pET vectors from which it differs by a single nucleotides that was changed to disrupt the NdeI cloning site.

Usage and Biology

Our team used this RBS to drive the expression of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum (BBa_K2448021) under the control of pTacI promoter (BBa_K2448033)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None