Composite

Part:BBa_K2443026

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)


EF-G optimized for expression in E. coli

Elongation factor G is a prokaryotic translocase and GTPase responsible for catalyzing and coordinating the movement of tRNA and mRNA through the ribosome. It has been Codon optimized for use in Escherichia coli and contains an N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
    Illegal AgeI site found at 629
    Illegal AgeI site found at 1268
    Illegal AgeI site found at 1330
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 1973
    Illegal AgeI site found at 2039
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None