RBS

Part:BBa_K2417009

Designed by: Erica Stewart   Group: iGEM17_Sydney_Australia   (2017-10-25)


Extended ribosome binding site

pET vectors were constructed in the 1980s, and are designed for either transcription or translation efficient purposes. The Extended Ribosome Binding Site originates from the vector pET15b, and is sourced from the phage T7 major capsid protein, used for its high ribosomal recruitment efficiency.

References: Novagen pET Plasmid Manual - TB055 8th Edition 02/99

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None