Part:BBa_K2371021

coding sequence of sgRNA for EML4-ALK Variant B 70

The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) is a fusion gene resulting from chromosome inversion and was identified in nonsmall cell lung cancer(NSCLC).The EML4-ALK fused gene has two variants (A and B).Using computer stimulation, we selected seven sgRNA binding sites from all the combination of possible sites. Four sgRNA binding sites on Variant A and three sgRNA binding sites on Variant B.

Figure 1. The four sgRNA binding sites of fusion gene EML4-ALK Variant A. We designed four sgRNA binding sites in target gene – sgRNA23, sgRNA33, sgRNA83 and sgRNA93. The number of them indicates the location of their first base pair in target gene. The combination of sgRNA binding sites will be based on the rule that a pair of sgRNA should be distributed in EML4 or ALK area separately or one of them located on the connecting area of the EML4-ALK fusion gene.

Figure 2. The three sgRNA binding sites of fusion gene EML4-ALK Variant B. We designed three sgRNA binding sites in target gene – sgRNA70, sgRNA126 and sgRNA147. The number of them indicates the location of their first base pair in target gene. The combination of sgRNA binding sites will be based on the rule that a pair of sgRNA should be distributed in EML4 or ALK area separately or one of them located on the connecting area of the EML4-ALK fusion gene.

This part is coding sequence of sgRNA VB 70 mentioned above.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]