Part:BBa_K2246006

anti-GFP siRNA

siRNA sequence for silencing protein green flourescent protein (GFP) gene. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for green flourescent protein (GFP) gene. Designed to be used as negative control during RNA interference experiments. In previous studies involving RNA interferance in D.citri, a dsRNA for GFP was used as negative control, see Taning, Andrade, Hunter, Christiaens & Smagghe (2016), El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny (2013) and Yu, Gowda & Killiny (2017). El-Shesheny I, Hajeri S, El-Hawary I, Gowda S, Killiny N .(2013). Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality. PLoS ONE 8(5): e65392. doi:10.1371/journal.pone.0065392 Taning C., Andrade E., Hunter W., Christiaens O. & Smagghe G. (2016). Asian Citrus Psyllid RNAi Pathway –RNAi evidence. Scientific Reports. DOI: 10.1038/srep38082 Yu X., Gowda S. & Killiny N. (2017). Double-stranded RNA delivery throughsoaking mediates silencing of the muscleprotein 20 and increases mortality to the Asiancitrus psyllid, Diaphorina citri. Pest Management Science. DOI 10.1002/ps.4549

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]